Carbon-14: Difference between revisions
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===Carbon-14 Tracing=== | ===Carbon-14 Tracing=== | ||
In her book ''Finding The Mother Tree'', Suzanne Simard used this method to track the path of carbon through the trees' root systems, and prove that trees exchange <sup>14</sup>C through micorrhizal pathways [60]. | |||
[[File:HowTreesTalk.jpg|376px|thumb|right|Suzanne Simard's drawing of carbon tracking through tree root systems [30]]] | [[File:HowTreesTalk.jpg|376px|thumb|right|Suzanne Simard's drawing of carbon tracking through tree root systems [30]]] | ||
==Analysis methods== | ==Analysis methods== | ||
Cation exchange resin (CER) extraction is currently considered the best method for accurately extracting EPS from soils.<ref name=Redmile-Gordon_2014>Redmile-Gordon, M.A., Brookes, P.C., Evershed, R.P., Goulding, K.W.T., Hirsch, P.R., 2014. Measuring the soil-microbial interface: Extraction of extracellular polymeric substances (EPS) from soil biofilms. Soil Biology and Biochemistry 72, 163–171.</ref>This method shows the highest efficency with the least amount of modification to the original EPS composition when compared to other methods. This method works by replacing cations in the material being sampled. This destabilizes the structure and allows for separation through filtration and/or centrifuge. | Cation exchange resin (CER) extraction is currently considered the best method for accurately extracting EPS from soils.<ref name=Redmile-Gordon_2014>Redmile-Gordon, M.A., Brookes, P.C., Evershed, R.P., Goulding, K.W.T., Hirsch, P.R., 2014. Measuring the [[soil]]-microbial interface: Extraction of extracellular polymeric substances (EPS) from soil biofilms. Soil Biology and Biochemistry 72, 163–171.</ref>This method shows the highest efficency with the least amount of modification to the original EPS composition when compared to other methods. This method works by replacing cations in the material being sampled. This destabilizes the structure and allows for separation through filtration and/or centrifuge. | ||
Revision as of 13:40, 30 March 2025
Overview
Carbon-14, also referred to as 14C or radiocarbon, is one of the three naturally occurring isotopes of carbon in nature. 14C is unstable, and has a half-life of 5700 +/- 30 years [10]. The other two forms of carbon are 12C, which is what makes up 98% of all carbon in the atmosphere, and 13C, which makes up around 1% of atmospheric carbon. Both 12C and 13C are stable isotopes, while 14C is unstable. It only occurs in trace amounts naturally, around 1 14C atom for every 7.54 x 1011 atoms of 12C in the atmosphere. Within the context of ecology, 14C is used in two main processes: dating organic materials and tracing carbon pathways within ecosystems.
Uses
Tree Ring Dating
14C is found in the atmosphere, and although it occurs in trace amounts, it will be absorbed by trees like any other form of carbon. The science of dendochronology heavily relies on 14C. Scientists are able to compare the tree rings of similar species growing in similar environments and match up their ages based on their rings [50]. From there, the 14C content in each tree ring can be matched to the tree's age and year it was growing. Since the amount of 14C in the atmosphere changes over time, scientists are also able to use the 14C content in each tree ring to relate it to the atmospheric conditions at the time the tree was growing. Because of all of this data, it is possible to know the tree-ring chronologies for the past 12,400 years [40].
Carbon-14 Tracing
In her book Finding The Mother Tree, Suzanne Simard used this method to track the path of carbon through the trees' root systems, and prove that trees exchange 14C through micorrhizal pathways [60].
Analysis methods
Cation exchange resin (CER) extraction is currently considered the best method for accurately extracting EPS from soils.[1]This method shows the highest efficency with the least amount of modification to the original EPS composition when compared to other methods. This method works by replacing cations in the material being sampled. This destabilizes the structure and allows for separation through filtration and/or centrifuge.